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BiochemistryNucleic Acids
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Q52000. All of the following statements about puromycin are true except

A.It is an alanyl tRNA analogue
B.It causes premature termination of protein
C.It inhibits protein synthesis in prokaryotes
D.It inhibits protein synthesis in eukaryotes

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52001. Leucine zipper motif is seen in some helical proteins when leucine residues appear at every

A.3rd position
B.5th position
C.7th position
D.9th position

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52002. Zinc finger motif is formed in some proteins by binding of zinc to

A.Two cysteine residues
B.Two histidine residues
C.Two arginine residues
D.Two cysteine and two histidine residues or two

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q52003. Restriction endonucleases are present in

A.Viruses
B.Bacteria
C.Eukaryotes
D.All of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52004. Restriction endonucleases split

A.RNA
B.Single stranded DNA
C.Double stranded DNA
D.DNA-RNA hybrids

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52005. Restriction endonucleases can recognise

A.Palindromic sequences
B.Chimeric DNA
C.DNA-RNA hybrids
D.Homopolymer sequences

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52006. All of the following statements about restriction endonucleases are true except:

A.They are present in bacteria
B.They act on double stranded DNA
C.They recognize palindromic sequences
D.They always produce sticky ends

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q52007. Which of the following is a palindromic sequence

A.(B)
B.(D)

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52008. In sticky ends produced by restriction endonucleases

A.The 2 strands of DNA are joined to each other
B.The DNA strands stick to the restriction
C.The ends of a double stranded fragment are
D.The ends of a double stranded fragment are

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q52009. All of the following may be used as ex- pression vectors except

A.Plasmid
B.Bacteriophage
C.Baculovirus
D.E. coli

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52010. A plasmid is a

A.Single stranded linear DNA
B.Single stranded circular DNA
C.Double stranded linear DNA
D.Double stranded circular DNA

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52011. Fragments of DNA can be identified by the technique of

A.Western blotting
B.Eastern blotting
C.Northern blotting (D) Southern blotting

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52012. A particular RNA in a mixture can be identified by

A.Western blotting
B.Eastern blotting
C.Northern blotting (D) Southern blotting

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52013. A radioactive isotope labeled cDNA probe is used in

A.Southern blotting
B.Northern blotting
C.Both (A) and (B)
D.None of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52014. An antibody probe is used in

A.Southern blotting
B.Northern blotting
C.Western blotting
D.None of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52015. A particular protein in a mixture can be detected by A) Southern blotting

A.Northern blotting
B.Western blotting
C.None of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52016. The first protein synthesized by recom- binant DNA technology was

A.Streptokinase
B.Human growth hormone
C.Tissue plasminogen activator
D.Human insulin

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52017. For production of eukaryotic protein by recombinant DNA technology in bacteria, the template used is

A.Eukaryotic gene
B.hnRNA
C.mRNA
D.All of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52018. Monoclonal antibodies are prepared by cloning

A.Myeloma cells
B.Hybridoma cells
C.T-Lymphocytes
D.B-Lymphocytes

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q52019. Myeloma cells are lacking in

A.TMP synthetase
B.Formyl transferase
C.HGPRT
D.All of these

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MCQs in BiochemistryNucleic Acids
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