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BiochemistryNucleic Acids
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Q51860. For unwinding of double helical DNA,

A.Energy is provided by ATP
B.Energy is provided by GTP
C.Energy can be provided by either ATP or GTP
D.No energy is required

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51861. Helicase and DNAB protein cause

A.Rewinding of DNA and require ATP as a
B.Rewinding of DNA but do not require any
C.Unwinding of DNA and require ATP as a
D.Unwinding of DNA but do not require any

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q51862. The unwound strands of DNA are held apart by

A.Single strand binding protein
B.Double strand binding protein
C.Rep protein
D.DNAA protein

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51863. Deoxyribonucleotides are added to RNA primer by

A.DNA polymerase I
B.DNA polymerase II
C.DNA polymerase III holoenzyme
D.All of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51864. Ribonucleotides of RNA primer are re- placed by deoxyribonucleotides by the enzyme:

A.DNA polymerase I
B.DNA polymerase II
C.DNA polymerase III holoenzyme
D.All of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51865. DNA fragments are sealed by

A.DNA polymerase II
B.DNA ligase
C.DNA gyrase
D.DNA topoisomerase II

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51866. Negative supercoils are introduced in DNA by

A.Helicase
B.DNA ligase
C.DNA gyrase
D.DNA polymerase III holoenzyme

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51867. Reverse transcriptase activity is present in the eukaryotic:

A.DNA polymerase α
B.DNA polymerase γ
C.Telomerase
D.DNA polymerase II

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51868. DNA polymerase III holoenzyme possesses

A.Polymerase activity
B.3’→5’ Exonuclease activity
C.5’→3’ Exonuclease and polymerase activities
D.3’→5’ Exonuclease and polymerase activities

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51869. DNA polymerase I possesses

A.Polymerase activity
B.3’→5’ Exonuclease activity
C.5’→3’ Exonuclease activity
D.All of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51870. 3’→5’ Exonuclease activity of DNA polymerase I

A.Removes ribonucleotides
B.Adds deoxyribonucleotides
C.Corrects errors in replication
D.Hydrolyses DNA into mononucleotides

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51871. All of the following statements about RNA-dependent DNA polymerase are true except:

A.It synthesizes DNA using RNA as a template
B.It is also known as reverse transcriptase
C.It synthesizes DNA in 5’→3’ direction
D.It is present in all the viruses

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q51872. Reverse transcriptase catalyses

A.Synthesis of RNA
B.Breakdown of RNA
C.Synthesis of DNA
D.Breakdown of DNA

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51873. DNA A protein can bind only to

A.Positively supercoiled DNA
B.Negatively supercoiled DNA
C.Both (A) and (B)
D.None of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51874. DNA topoisomerase I of E. coli catalyses

A.Relaxation of negatively supercoiled DNA
B.Relaxation of positively supercoiled DNA
C.Conversion of negatively supercoiled DNA
D.Conversion of double helix into supercoiled

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MCQs in Biochemistry
BiochemistryNucleic Acids
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Q51875. In mammalian cell cycle, synthesis of DNA occurs during

A.S phase
B.G1 phase
C.Mitotic Phase
D.G2 phase

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51876. Melting temperature of DNA is the tempera- ture at which

A.Solid DNA becomes liquid
B.Liquid DNA evaporates
C.DNA changes from double helix into
D.Native double helical DNA is denatured

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51877. Melting temperature of DNA is increased by its

A.A and T content
B.G and C content
C.Sugar content
D.Phosphate content

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51878. Buoynat density of DNA is increased by its

A.A and T content
B.G and C content
C.Sugar content
D.None of these

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MCQs in BiochemistryNucleic Acids
BiochemistryNucleic Acids
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Q51879. Relative proportions of G and C versus A and T in DNA can be determined by its

A.Melting temperature
B.Buoyant density
C.Both (A) and (B)
D.None of these

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MCQs in BiochemistryNucleic Acids
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